Coding

Part:BBa_K5214002:Design

Designed by: Shan Dong   Group: iGEM24_Bioplus-Shanghai   (2024-09-09)


Cytochrome P450 Subfamily 98A Monooxygenase-75 (SmCYP98A75)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 441
    Illegal BsaI.rc site found at 1431
    Illegal SapI site found at 390


Design Notes

Be aware of the enzymatic sites of the sequence.


Source

A segment of cDNA sequence from S. miltiorrhiza.

References